AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JARAK MERAH (Jatropha gossypiifolia) ASAL KABUPATEN BANTAENG

  • Nurzadrina Wahyuddin Sekolah Tinggi Ilmu Farmasi Makassar
  • Ismail Ismail Sekolah Tinggi Ilmu Farmasi Makassar
  • Harlyanti Muthma'innah Mashar Poltekkes Kemenkes Palangka Raya
  • Dali Jurusan Keperawatan, Poltekkes Kemenkes Kendari
Keywords: Red castor, Jatropha gossypiifolia, Antioxicant, DPPH, IC50

Abstract

Background: In the last few decades there has been an increase in cases of chronic diseases and premature aging which are some of the key factors in influencing public health. This condition is caused by various causes, but the main factor is the number of free radicals that exceed the body's capacity to neutralize them. Antioxidants have an important role as health protective factors because they can neutralize and protect cells from the presence of free radicals. Red castor leaves have been reported to have good activity as an antioxidant. The chemical content of this plant is anthraquinone, flavonoid, saponin, tannin and terpenoid. Objective: This study aimed to evaluate the antioxidant activity of red jatropha leaf extract using the DPPH method. Methods Samples were extracted using 70% ethanol by maceration method. The antioxidant activity test was carried out using the DPPH method (1,1-diphenyl-2-picrylhydrazyl) using a comparison of Vitamin C. Each test used a variation of the concentration of the solution. For red jatropha leaf extract using concentration variations of 20, 40, 60, 80 and 100 g/ml, while for vitamin C using concentration variations of 10, 15, 20, 25, and 30 g/ml. The absorbance of each test solution was measured using a spectrophotometer at a wavelength of 515 nm. The results are reported as IC50 values. Results: The results showed that the IC50 values ​​of red jatropha leaf extract and vitamin C were 47.81 and 14.99 g/ml, respectively. This value indicates that the Chinese jatropha leaf extract has a very strong antioxidant activity (IC50 <50 g/ml). Conclusion:  Red jatropha leaf extract showed very strong antioxidant activity with an IC50 value of 47.81 g/ml so it has great potential as an antioxidant that can be used for the development of traditional medicinal raw materials.

References

Andriani, D. & Murtisiwi, L. 2020. Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH. Pharmacon: Jurnal Farmasi Indonesia; 17(1); 70–76.
Asep, S. et al. 2017. Anticancer activity of jatrophone an isolated compound from Jatropha gossypifolia plant against hepatocellular cancer cell Hep G2 1886. Biomedical and Pharmacology Journal; 10(2); 667–673.
Bahriul, P., Rahman, N. & Diah, A. W. M. 2014. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzyngium polyanthum) Dengan Metode DPPH. Jurnal Akademika Kimia; 3(3); 368–374.
Bhattacharya, S., Debnath, S., Das, P., & Saha, A. K. 2018. Antioxidant Activity Of Fungal Endophyte Aspergillus sydowiis Ananus Comosus L. Isolated from Ananus comosus L. International Journal of Current Advanced Research; 7(4); 11466–11469.
Bourhia, M. et al. 2019. Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species. Evidence-based Complementary and Alternative Medicine.
Cabello-Verrugio, C. et al. 2018. Oxidative stress in disease and aging: Mechanisms and therapies 2018. Oxidative Medicine and Cellular Longevity; () ;1–3.
Dubey, R., Rajhans, S. & Mankad, A. U. 2020. Phytochemicals of Jatropha gossypiifolia (Linn.): A Review. International Journal of Innovative Science and Research Technology; 5(3); 904–911.
Félix-silva, J. et al. 2014. A Review of Traditional Uses , Phytochemistry , Pharmacology , and Toxicology of This Medicinal Plant. Evidence-Based Complementary and Alternative Medicine; (); 1–32.
Félix-Silva, J. et al. 2014. Jatropha gossypiifolia L. (Euphorbiaceae): A review of traditional uses, phytochemistry, pharmacology, and toxicology of this medicinal plant. Evidence-based Complementary and Alternative Medicine.
Finkel, T. & Holbrook, N. J. .2000. Oxidants, oxidative stress and the biology of ageing. Nature; 408(6809); 239–247.
Fitria, A., Nur Upziah, D. & Lakna Widya Lestari, S. 2016. Studi Aktivitas Dan Analisis Kandungan Senyawa Antioksidan Batang Jatropha Multifida L. Jurnal Ilmiah Farmasi; 12(2); 15–26.
Mahdi-Pour, B. et al. 2012. Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pacific Journal of Tropical Biomedicine; 2(12); 960–965.
Mashar, H. M. & Annah, I. 2020. Cytotoxicity of Kelakai (Stenochlaena palustris) Extract to MCF-7 Breast Cancer Cell. Jurnal Fitofarmaka Indonesia; 7(3); 5–9.
Russo, G. et al. 2012. Oxidative Stress and Diseases. Oxidative Stress and Diseases; (); 757–772.
Sharifi-Rad, M. et al. 2020. Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Frontiers in Physiology; 11(7); 1–21.
Sekhar, T.C., & Anju, G. 2014. Antioxidant Activity by DPPH Radical Scavenging Method of Ageratum conyzoides Linn. Leaves. American Journal of Ethnomedicine; 1(4); 244–249.
Tania, P. O. A. 2018. Free Radical, Oxidative Stress and Its Roles on Inflammatory Response. Berkala Kedokteran; 14(2); 179.
Torokano, S., Khumaidi, A. & Nugrahani, A. W. 2018. Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Merah (Jatropha gossypifolia) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Natural Science: Journal of Science and Technology; 7(1); 117–126.
Utami, R. R. et al. 2017. Aktivitas Antioksidan Kulit Biji Kakao dari Hasil Penyangraian Biji Kakao Kering pada Derajat Ringan, Sedang dan Berat. Agritech; 37(1); 89.
Published
2022-03-31
How to Cite
Wahyuddin, N., Ismail, I., Mashar, H. M., & Dali. (2022). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JARAK MERAH (Jatropha gossypiifolia) ASAL KABUPATEN BANTAENG. Jurnal Kefarmasian Akfarindo, 29-34. https://doi.org/10.37089/jofar.vi0.115
Section
Artikel