PERAN miRNA-21 PENEKAN GEN PTEN PENSIYALAN PI3K/AKT PADA KANKER KOLOREKTAL

  • Ismail Ismail Sekolah Tinggi Ilmu Farmasi Makassar
  • Arisanti Pulio Sekolah Tinggi Ilmu Farmasi Makassar
  • Elvin Bura Sekolah Tinggi Ilmu Farmasi Makassa
  • Selfiana Sekolah Tinggi Ilmu Farmasi Makassar
  • Nur Mutmainnah Sekolah Tinggi Ilmu Farmasi Makassar
Keywords: miRNA-21, Cancer Colorectal, Gene PTEN, StarMir

Abstract

Colorectal cancer is one of the leading causes of death in the world caused by various factors such as genetic mutations, epigenetics, dysregulation of communication signaling pathways and gut microbiota.  In the presence of genetic, epigenetic mutations, there is a loss of expression and function of PTEN (Phosphatase and TENsin homologues) resulting in continued activation of the PI3K/Akt intracellular signaling cascade involved in colorectal carcinogenesis.  The solution to this problem is to use miRNA-21.  which plays an important role by suppressing the biological marker PTEN gene that is useful for diagnosis and treatment in colorectal cancer.  The aim of this study was to determine the specifi c binding of miRNA-21 with the PTEN gene that plays a role in colorectal cancer based on the AGhybrid and Logistic Probability values.  Complete sequences of PTEN genes were obtained from FASTA NCBI and CDS data were obtained from Refseq.  Prediction of the binding of mature miRNA-21 with the PTEN gene was carried out using the StarMir Sfold 2.2 website.  The predicted results of miRNA-21 binding with 1 target obtained 296 binding models.  Specific binding was seen from AGhybrid -23.600 and logistic probability 0.818, there was a binding between PTEN gene binding and miR-21-5p at seedless CDS position 3627-3653 as evidenced by the lowest AGhybrid value of all bindings that occurred.

References

Fadaka, A.O. Klein, A. Pretorius, A. 2019. In Silico identification of microRNAs as candidate colorectal cancer biomaker. Tumor Biology. 1-15
Iseki, Y. Shibutani, M. Maeda, K. Nagahara, H. Fukuoka, T. Ikeya, T. Matsutani, S. Kimura, K. Toyokawa, T. Amano, R. Tanaka, H. Muguruma, K. Hirakawa, K. Ohira, M.. 2016. Prognostic significance of microRNA-21 expession in patients with Unresectable Metastatic colon cancer. Anticancer Research 36:5145-5152
Makatita, FA. Wardhani, R. Nuraini. Riset in-Silico dalam Pengembangan Sains di Bidang Pendidikan, Studi Kasus: Analisis Potensi Cendana Sebagai Agen Anti-Aging. Jurnal ABDI Vol.2 No.1 Januari 2020.
Molinari,F. and Frattini,M. 2014. Function and regulation of the PTEN gene in colorectal cancer. Frontiers in ONCOLOGY. 3:326.
Padang, M.S dan Rotty, L. 2019. Adnekarsinoma kolon: Laporan kasus. e-Clinic. 8(2):229-236
Rennie, W., Liu C., Carmack, S., Wolenc, A., Kanoria, S., Lu, J., et al., 2014 Starmir: A Web Server for Prediction of micoRNA Binding Sites, Nucleic Acid Research, 42: 114-118
Rennie, W., Kanoria, S., Liu, C., Mallick, B., Long, D., Wolenc, A., et al., 2016, STarMirDB: A Database of MicroRNA Binding Sites, RNA Biology, 13: 554–560
Rompis, A. Y. dan Ni Nyoman. A. D. 2020. Aspek Genetik Kanker kolorektal. Jurnal Sains Kesehatan. 2(3)
Sayuti, M dan Nouva. 2019. Kanker kolorektal. Jurnal Averrous, 5(2)
Sun, Y., Yahong X., Jie X., and Lu, J W. 2015. Role of TM4SF1 in regulating breast cancer cell migration and apoptosis through PI3K/AKT/mTOR pathway. International Journal of Clinical and experimental pathology 8 (8), 9081, 2015.
Tamba,E. 2012. Karsinogenesis kanker Kolorektal, hubungannya dengan Diet dan Mikroflora usus. Jurnal Kedokteran Meditek. 18(48)
WHO,2018. Latest globl cancer data: Cancer burden rises to 18,1 million new cases and 9,6 million cancer deaths in 2018.
Wang, X., Tian, J., Cui, P., Mastriano, S., Zhang, D., Zhao, H., et al., 2019, microRNA Seedless Sites Attenuate Strong-Seed-SideMediate Target Repsession, BioRxiv, http://doi.org/10.1101/837682
Wu, Yiying. Song, Yi. Xiong, Y. Wang, X. Xu, Ke. Han, Bi. Bai, Y. Li, L. Zhang, Y. Zhou, L. 2017. MicroRNA-21 (Mir-21) Promotes Cell Growth and Invasion by Repressing Tumor Suppressor PTEN in Colorectal Cancer. Cell PhysiolBiochem, 43:945-958.
Published
2022-09-30
How to Cite
Ismail, I., Pulio, A., Bura, E., Selfiana, & Mutmainnah, N. (2022). PERAN miRNA-21 PENEKAN GEN PTEN PENSIYALAN PI3K/AKT PADA KANKER KOLOREKTAL. Jurnal Kefarmasian Akfarindo, 88-93. https://doi.org/10.37089/jofar.vi0.121
Section
Artikel